Wattananarong Markphan

Environmental Program, Faculty of Science and Technology, Nakhon Si Thammarat Rajabhat University, Nakhon Si Thammarat, Thailand

Peerawat Khongkliang

The Joint Graduate School of Energy and Environment, King Mongkut’s University of Technology Thonburi, Bangkok, Thailand

Wantanasak Suksong

Excellent Center of Waste Utilization and Management (ECoWaste), Pilot Plant Development and Training Institute (PDTI), King Mongkut’s University of Technology Thonburi, Bangkok, Thailand

Wisarut Tukanghan

Excellent Center of Waste Utilization and Management (ECoWaste), Pilot Plant Development and Training Institute (PDTI), King Mongkut’s University of Technology Thonburi, Bangkok, Thailand

Chonticha Mamimin

Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, Thailand

DOI: https://doi.org/10.14456/apst.2025.12

Keywords: Organic fraction municipal solid waste Green waste Co-digestion Methane production Microbial community


Abstract

Biogas production from the organic fraction of municipal solid waste (OFMSW) is often hindered by the risk of acidic failure at high loadings due to its readily biodegradable nature. This study explored the potential of enhancing methane production through the co-digestion of OFMSW with green waste (GW) in anaerobic fermentation. By evaluating the impact of the carbon-to-nitrogen (C/N) ratio on digestion efficiency, we compared the performance of individual and co-digestion processes. The results demonstrated that co-digestion significantly outperformed individual digestion, achieving a maximum methane yield of 420 L/kgVS with an optimal OFMSW ratio of 2:1. At 10% total solids (TS) loading, this ratio led to a 72% improvement in mass and energy balance compared to the digestion of OFMSW or GW alone. Microbial community analysis revealed that Clostridium sp. was the dominant bacterium in the co-digestion process, while Methanobacterium sp. was the predominant archaea, both playing crucial roles in methane production. The co-digestion of the readily biodegradable OFMSW with the more slowly degradable GW proved to be an effective strategy for enhancing methane yield and energy recovery. These findings indicate the viability of co-digestion as a robust approach for optimizing methane production and advancing sustainable waste management and renewable energy generation.


How to Cite

Markphan, W., Khongkliang, P., Suksong, W., Tukanghan, W., & Mamimin, C. (2025). Green waste addition to boosting solid-state anaerobic digestion of municipal solid waste for efficient methanogenesis and energy recovery. Asia-Pacific Journal of Science and Technology30(01), APST–30. https://doi.org/10.14456/apst.2025.12


References

Ashani PN, Shafiei M, Karimi K. Biobutanol production from municipal solid waste: Technical and economic analysis. Bioresour Technol. 2020;308:123267.

Prateep Na Talang R, Sirivithayapakorn S. Comparative analysis of environmental costs, economic return and social impact of national-level municipal solid waste management schemes in Thailand. J Clean Prod. 2022;343:131017

Chaianong A, Pharino C. How to design an area-based prioritization of biogas production from organic municipal solid waste? Evidence from Thailand. Waste Manag. 2022;138:243–252

Chuenwong K, Wangjiraniran W, Pongthanaisawan J, Sumitsawan S, Suppamit T. Municipal solid waste management for reaching net-zero emissions in ASEAN tourism twin cities: A case study of Nan and Luang Prabang. Heliyon. 2022;8(8):e10295.

Ali G, Nitivattananon V, Abbas S, Sabir M. Green waste to biogas: Renewable energy possibilities for Thailand’s green markets. Renew Sustain Energy Rev. 2012;16(7):5423–5429.

Ascher S, Watson I, Wang X, You S. Township-based bioenergy systems for distributed energy supply and efficient household waste re-utilisation: Techno-economic and environmental feasibility. Energy (Oxf). 2019;181:455–467.

González-Sánchez ME, Pérez-Fabiel S, Wong-Villarreal A, Bello-Mendoza R, Yañez-Ocampo G. Residuos agroindustriales con potencial para la producción de metano mediante la digestión anaerobia. Rev Argent Microbiol. 2015;47(3):229–235.

Balat M, Balat H. Biogas as a renewable energy source—A review. Energy Sources Recovery Util Environ Eff. 2009;31(14):1280–1293

Pongsopon M, Woraruthai T, Anuwan P, Amawatjana T, Tirapanampai C, Prombun P, et al. Anaerobic co-digestion of yard waste, food waste, and pig slurry in a batch experiment: An investigation on methane potential, performance, and microbial community. Bioresour Technol Rep. 2023;21:101364.

Suksong W, Kongjan P, Prasertsan P, Imai T, O-Thong S. Optimization and microbial community analysis for production of biogas from solid waste residues of palm oil mill industry by solid-state anaerobic digestion. Bioresour Technol. 2016;214:166–174

Prasertsan P, Khangkhachit W, Duangsuwan W, Mamimin C, O-Thong S. Direct hydrolysis of palm oil mill effluent by xylanase enzyme to enhance biogas production using two-steps thermophilic fermentation under non-sterile condition. Int J Hydrogen Energy. 2017;42(45):27759–27766.

APHA. Standard methods for the examination of water and wastewater. 22nd ed. Washington, USA: American Public Health Association. 2012.

Van Soest PJ, Robertson JB, Lewis BA. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. J Dairy Sci. 1991;74(10):3583–3597.

Yan Z, Song Z, Li D, Yuan Y, Liu X, Zheng T. The effects of initial substrate concentration, C/N ratio, and temperature on solid-state anaerobic digestion from composting rice straw. Bioresour Technol. 2015;177:266–273.

O-Thong S, Boe K, Angelidaki I. Thermophilic anaerobic co-digestion of oil palm empty fruit bunches with palm oil mill effluent for efficient biogas production. Appl Energy. 2012;93:648–654

Markphan W, Mamimin C, Suksong W, Prasertsan P, O-Thong S. Comparative assessment of single-stage and two-stage anaerobic digestion for biogas production from high moisture municipal solid waste. PeerJ. 2020;8:e9693.

Zamri MFMA, Hasmady S, Akhiar A, Ideris F, Shamsuddin AH, Mofijur M, et al. A comprehensive review on anaerobic digestion of organic fraction of municipal solid waste. Renew Sustain Energy Rev. 2021;137:110637.

Chen JL, Ortiz R, Steele TWJ, Stuckey DC. Toxicants inhibiting anaerobic digestion: a review. Biotechnol Adv. 2014;32(8):1523–1534.

Liu G, Zhang R, El-Mashad HM, Dong R. Effect of feed to inoculum ratios on biogas yields of food and green wastes. Bioresour Technol. 2009;100(21):5103–5108.

Paritosh K, Mathur S, Pareek N, Vivekanand V. Feasibility study of waste (d) potential: co-digestion of organic wastes, synergistic effect and kinetics of biogas production. Int J Environ Sci Technol. 2018;15(5):1009–1018

Ali Shah F, Mahmood Q, Maroof Shah M, Pervez A, Ahmad Asad S. Microbial ecology of anaerobic digesters: the key players of anaerobiosis. Sci World J. 2014;2014:183752.

Hania WB, Bouanane-Darenfed A, Cayol J-L, Ollivier B, Fardeau M-L. Reclassification of Anaerobaculum mobile, Anaerobaculum thermoterrenum, Anaerobaculum hydrogeniformans as Acetomicrobium mobile comb. nov., Acetomicrobium thermoterrenum comb. nov. and Acetomicrobium hydrogeniformans comb. nov., respectively, and emendation of the genus Acetomicrobium. Int J Syst Evol Microbiol. 2016;66(3):1506–1509.

Wainaina S, Lukitawesa, Kumar Awasthi M, Taherzadeh MJ. Bioengineering of anaerobic digestion for volatile fatty acids, hydrogen or methane production: A critical review. Bioengineered. 2019;10(1):437–458.

Panschin I, Becher M, Verbarg S, Spröer C, Rohde M, Schüler M, et al. Description of Gramella forsetii sp. nov., a marine Flavobacteriaceae isolated from North Sea water, and emended description of Gramella gaetbulicola Cho et al. 2011. Int J Syst Evol Microbiol. 2017;67(3):697–703.

Rabii A, Aldin S, Dahman Y, Elbeshbishy E. A review on anaerobic co-digestion with a focus on the microbial populations and the effect of multi-stage digester configuration. Energies. 2019;12(6):1106.

aldez-Vazquez I, Morales AL, Escalante AE. History of adaptation determines short-term shifts in performance and community structure of hydrogen-producing microbial communities degrading wheat straw. Microb Biotechnol. 2017;10(6):1569–1580.

Braga JK, Abreu AA, Motteran F, Pereira MA, Alves MM, Varesche MBA. Hydrogen production by Clostridium cellulolyticum a cellulolytic and hydrogen-producing bacteria using sugarcane bagasse. Waste Biomass Valorization. 2019;10(4):827–837.

Ratti RP, Delforno TP, Okada DY, Varesche MBA. Bacterial communities in thermophilic H2-producing reactors investigated using 16S rRNA 454 pyrosequencing. Microbiol Res. 2015;173:10–17.

Joung Y, Kim H, Jang T, Ahn T-S, Joh K. Gramella jeungdoensis sp. nov., isolated from a solar saltern in Korea. J Microbiol. 2011;49(6):1022–1026.

Martins M, Mourato C, Pereira IAC. Desulfovibrio vulgaris growth coupled to formate-driven H2 production. Environ Sci Technol. 2015;49(24):14655–14662.