Nittiyaphorn Sotapong
Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand
Mallika B. Kongkeitkajorn
Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, Thailand
Atcha Oraintara
Department of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand
DOI: https://doi.org/10.14456/apst.2022.25
Keywords: 1,3-Propanediol Glycerol utilizing bacteria Klebsiella pneumoniae 1,3-propanediol oxidoreductase
Abstract
Glycerol is a by-product waste from biodiesel production, which can be converted by bacteria to other value-added products. One of these products is 1,3-propanediol (1,3-PDO), an important substrate for synthesis of biopolymer and many others. This research aims to isolate bacteria that can utilize glycerol as a sole carbon source and examine the 1,3-PDO production ability of these isolates using glycerol as a substrate. Bacterial isolates were obtained from soil near biodiesel plant by culturing in mineral salt medium containing glycerol. Growth of selected isolates in a medium containing glycerol as a sole carbon source were monitored and compared with reference bacterial strains. It was observed that 2 isolates grew better than reference strains according to optical density value at 600 nm. Both isolates were identified as Klebsiella pneumoniae by 16S rRNA gene sequencing. The isolate 5-3, renamed as K. pneumoniae KKU5 (Kp KKU5) was used for further study. The dhaT gene of Kp KKU5 shared 99% identity with those of K. pneumoniae (CP025630.1). To examine the ability of Kp KKU5 as a 1,3-PDO producer, its fermentation broth containing glycerol was analyzed by high-performance liquid chromatography. Under batch fermentation conditions with 20 g/L initial glycerol, Kp KKU5 produced 0.375 g/L of 1,3-PDO after 7 days. Despite its low production of 1,3-propanediol, we have demonstrated that Kp KKU5 was able to utilize glycerol as a sole carbon source. This study might provide some useful information to contribute in the research area of 1,3-PDO production, that adds value to glycerol waste from biodiesel production.
How to Cite
Sotapong, N., Kongkeitkajorn, M. B., & Oraintara, A. (2022). Isolation of bacteria utilizing glycerol as a sole carbon source for potential production of 1,3-propanediol. Asia-Pacific Journal of Science and Technology, 27(02), APST–27. https://doi.org/10.14456/apst.2022.25
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