The effect of methanolic extract of Peronema canescens Jack. on HCT116 colorectal cancer cell line: In silico and in vitro approaches

Muhammad Priyadi

Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Universitas Palangka Raya, Palangka Raya, Central Kalimantan, Indonesia

Rian Ka Praja

Department of Microbiology, Faculty of Medicine, Universitas Palangka Raya, Palangka Raya, Central Kalimantan, Indonesia

Rizki Rachmad Saputra

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Palangka Raya, Palangka Raya, Central Kalimantan, Indonesia

Reny Rosalina

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Palangka Raya, Palangka Raya, Central Kalimantan, Indonesia

Decenly

Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Palangka Raya, Palangka Raya, Central Kalimantan, Indonesia

Dwi Hermayantiningsih

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Palangka Raya, Palangka Raya, Central Kalimantan, Indonesia

DOI: https://doi.org/10.14456/apst.2025.64

Keywords: Cytotoxicity Peronema canescens HCT116 EGFR

Abstract

Peronema canescens Jack. as a plant native to the forests of Kalimantan is empirically used by the Dayak community as a traditional medicine to treat various diseases, including its potential as an anticancer agent. Colorectal cancer, one of the cancers that many people suffer from, has the potential to be treated using herbal plants. In various phytochemical studies, the content of secondary metabolite compounds and pharmacological activity of P. canescens have been known in previous research, such as cytotoxic activity against cancer cells. The molecular target involved in the development of cancer is the epidermal growth factor receptor (EGFR), a protein responsible for the process of cancer. This study aimed to investigate the cytotoxic activity of P. canescens leaves on HCT116 colorectal cancer cells and molecular studies on EGFR. The methods used included cytotoxic tests using the neutral red (NR) test and molecular docking tests using Autodock Vina with the target protein EGFR. The results of the cytotoxic test of P. canescens leaf extract on HCT116 cells of 48-hour incubation had an IC50 value of 578.36 µg/mL and P. canescens leaf extract with 72-hour incubation was 400.88 µg/mL. The results of the molecular docking test showed that bioactive compound derivatives of P. canescens, pregnan20-one,3-(acetyloxy)-5,6:16,17-diepoxy-(3α,5α,6α,16α) and resibufogenin, have a high binding affinity to EGFR, with values of -7.9 and -7.8 kcal/mol each, comparable to osimertinib (-8.3 kcal/mol).

How to Cite

Priyadi, M., Praja, R. K. ., Saputra, R. R. ., Rosalina, R., Decenly, & Hermayantiningsih, D. . (2025). The effect of methanolic extract of Peronema canescens Jack. on HCT116 colorectal cancer cell line: In silico and in vitro approaches. Asia-Pacific Journal of Science and Technology, 30(04), APST–30. https://doi.org/10.14456/apst.2025.64

References

Arnold M, Sierra MS, Laversanne M, Soerjomataram I, Jemal A, Bray F. Global patterns and trends in colorectal cancer incidence and mortality. Gut. 2017;66(4):683–691.

Janani B, Vijayakumar M, Priya K, Kim JH, Prabakaran DS, Shahid M, et al. EGFR-based targeted therapy for colorectal cancer-promises and challenges. Vaccines. 2022;10(4):1–15.

Giftson JS, Jayanthi S, Nalini N. Chemopreventive efficacy of gallic acid, an antioxidant and anticarcinogenic polyphenol, against 1,2-dimethyl hydrazine induced rat colon carcinogenesis. Invest New Drugs. 2010;28(3):251–259.

Minker C, Duban L, Karas D, Järvinen P, Lobstein A, Muller CD. Impact of procyanidins from different berries on caspase 8 activation in colon cancer. Oxid Med Cell Longev. 2015;2015.

Grothey A, Venook AP. Optimizing adjuvant therapy for localized colon cancer and treatment selection in advanced colorectal cancer. J Natl Compr Cancer Netw. 2018;16(5S):611–15.

Lin X, Wang G, Liu P, Han L, Wang T, Chen K, et al. Gallic acid suppresses colon cancer proliferation by inhibiting SRC and EGFR phosphorylation. Exp Ther Med. 2021;21(6):1–11.

Martinelli E, Ciardiello D, Martini G, Troiani T, Cardone C, Vitiello PP, et al. Implementing anti-epidermal growth factor receptor (EGFR) therapy in metastatic colorectal cancer: challenges and future perspectives. Ann Oncol. 2020;31(1):30–40.

Piawah S, Venook AP. Targeted therapy for colorectal cancer metastases: a review of current methods of molecularly targeted therapy and the use of tumor biomarkers in the treatment of metastatic colorectal cancer. Cancer. 2019;125(23):4139–4147.

Saletti P, Molinari F, Dosso S De, Frattini M. EGFR signaling in colorectal cancer: a clinical perspective. Gastrointest Cancer Targets Ther. 2015;5:21–38.

Guo L, Huang S, Wang X. PUMA mediates the anti-cancer effect of osimertinib in colon cancer cells. Onco Targets Ther. 2017;10:5281–5288.

Troiani T, Napolitano S, Della Corte CM, Martini G, Martinelli E, Morgillo F, et al. Therapeutic value of EGFR inhibition in CRC and NSCLC: 15 years of clinical evidence. ESMO Open. 2016;1(5):1–14.

Jiang Y, Shi Y, Wang Z, Li H, Wang Z, He K, et al. Analysis of adverse drug reactions of Osimertinib in the 2nd‐line treatment of EGFR mutant advanced nonsmall cell lung cancer. Precis Med Sci. 2022;11(2):69–81.

Latief M, Fisesa AT, Sari PM, Tarigan IL. Aktivitas antiinflamasi ekstrak etanol daun sungkai (Peronema Canescens Jack) pada mencit terinduksi karagenan. J Farm Sains dan Prakt. 2021;7(2):2579–4558.

Fikriansyah M, Nelson N, Latief M, Tarigan IL. Anticancer activities of seven peronemins (A2, A3, B1, B2, B3, C1, and D1) from Peronema canescens Jack: A prediction studies. Chempublish J. 2023;7(1):54–63.

Amalia ID. Profil metabolit pada tingkat perkembangan daun sungkai (Peronema canescens Jack.) .Skripsi. Sriwijaya University; 2022.

Prahasti AE, Yuanita T, Rahayu RP. Computer aided drug discovery utilization in conservative dentistry. J Int Dent Med Res. 2022;15(2):899–903.

Ezhilarasan D, Ali D, Varghese R. Sesamol induces cytotoxicity via mitochondrial apoptosis in SCC-25 cells. Hum Exp Toxicol. 2021;40(12_suppl):S423–S433.

Copeland RA. The drug-target residence time model: A 10-year retrospective. Nat Rev Drug Discov. 2016;15(2):87–95.

Shultz MD. Two decades under the influence of the rule of five and the changing properties of approved oral drugs. J Med Chem. 2019;62(4):1701–1714.

Doan NQH, Truong TN, Nguyen PTV. Molecular docking studies of glycyrrhetinic acid derivatives as anti-colorectal cancer agents. Curr Comput Aided Drug Des. 2020;17(3):429–44.

Ciardiello F, Tortora G. EGFR antagonists in cancer treatment. New Engl J Med Rev.2008;358(11):1160–1174.

Pasaribu G, Hudiyono S, Budianto E, Cahyana AH, Pari G. In-vitro anticancer activity of Saurauia vulcani leaf extracts against human colon WiDr and HCT-116 cancer cells. Rasayan J Chem. 2021;2021(Special Issue):165–174.

Roberts PJ, Der CJ. Targeting the Raf-MEK-ERK mitogen-activated protein kinase cascade for the treatment of cancer. Oncogene. 2007;26(22):3291–3310.

Manning BD, Cantley LC. AKT/PKB signaling: Navigating downstream. Cell. 2007;129(7):1261–74.

Chan G, Gu S, Neel BG. Erk1 and Erk2 are required for maintenance of hematopoietic stem cells and adult hematopoiesis. Blood. 2013;121(18):3594–3598.

Vijayarathna S, Sasidharan S. Cytotoxicity of methanol extracts of Elaeis guineensis on MCF-7 and Vero cell lines. Asian Pac J Trop Biomed. 2012;2(10):826–829.

Çoruh N, Özdoğan N. Wild-growing Rosa heckeliana Tratt .: Phenolic constituents with cytotoxic and antioxidative properties. Turkish J Biol. 2017;41:195–212.

Madhuri BS, Bhaskar D, Sathyamurthy B. Invitro studies on the effect of Curcuma longa methanol extracts in colorectal HCT – 116 cell lines. Indo Am J Pharm Sci. 2017;4(9):3015–3023.

Abdelaziz MAA, Azger Dusthackeer VN, Peraman R. Anticancer activity of Grewia obtusa fruit on HCT-116, MCF-7, and HeLa tumour cells besides antitubercular activity. Indian J Pharm Educ Res. 2022;56(3):765–771.

Yang W, Chen X, Li Y, Guo S, Wang Z, Yu X. Advances in pharmacological activities of terpenoids. Nat Prod Commun. 2020;15(3): 1–13

Kamran S, Sinniah A, Abdulghani MAM, Alshawsh MA. Therapeutic potential of certain terpenoids as anticancer agents: a scoping review. Cancers (Basel). 2022;14(5):1100.

Ibrahim A, Siswandono, Bambang Prajogo EW. Cytotoxic activity of Peronema canescens Jack leaves on human cells: HT-29 and primary adenocarcinoma colon cancer. Pharmacogn J. 2021;13(6):1389–1396.