Oeij Anindita Adhika
Faculty of Medicine, Maranatha Christian University, Bandung 40164, West Java, Indonesia
Fen Tih
Faculty of Medicine, Maranatha Christian University, Bandung 40164, West Java, Indonesia
Keywords: Anti-inflammatory, Antioxidant, Lipopolysaccharide, RAW 264.7, Solanum lycopersicum
Abstract
Inflammation is considered a common primary contributor to multiple diseases; therefore, anti-inflammatory agents are important to reduce the associated risks. Tomato (Solanum lycopersicum L.) fruit extract systemic lupus erythematosus (SLE) contains numerous bioactive compounds. Therefore, SLE potential as an anti-inflammatory and antioxidant properties were evaluated in this study. SLE was extracted using the maceration method. NO scavenging activity of SLE was analyzed using Griess reagent. Cytotoxicity was performed using the MTS assay. LPS was used to induce RAW264.7 cells in the inflammation model. ELISA was used to measure PGE-2, TNF-α, and IL-1β protein levels, while colorimetry was utilized to examine total protein levels in LPS-induced RAW264.7 cells with SLE concentrations of 4 and 20 μg/mL. From this study, the IC₅₀ value was found to be 33.70 μg/mL, indicating that SLE has potential as an antioxidant, while 66.67 μg/mL SLE showed the greatest NO scavenging activity. SLE (4 and 20 μg/mL) was a safe concentration based on cytotoxic assay. SLE 20 μg/mL significantly reduced PGE-2, TNF-α, and IL-1β protein levels at the inflammation cells model. SLE showed its potential as an anti-inflammatory and antioxidant agent.
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