Effect of carbon, nitrogen and phosphorus on PHAs produced from Novosphingobium sp. THA_AIK7 and its structure

Sasina Promdee

Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani, Thailand

Atsadawut Areesirisuk

Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani, Thailand

Manoch Posung

Innovation and Medical Biotechnology Center (iMBC), Medical Life Sciences Institute, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand

Suthida Tuntigumthon

Innovation and Medical Biotechnology Center (iMBC), Medical Life Sciences Institute, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand

Panadda Dhepakson

Innovation and Medical Biotechnology Center (iMBC), Medical Life Sciences Institute, Department of Medical Sciences, Ministry of Public Health, Nonthaburi, Thailand

Tsuyoshi Imai

Graduate School of Sciences and Technology for Innovation Yamaguchi University, Yamaguchi, Japan

Jantima Teeka

Division of Biology, Faculty of Science and Technology, Rajamangala University of Technology Thanyaburi, Pathum Thani, Thailand

DOI: https://doi.org/10.14456/apst.2025.18

Keywords: Carbon Nitrogen Polyhydroxyalkanoates Phosphorus

Abstract

The production of polyhydroxyalkanoates (PHAs) by microorganisms usually occurs in response to environmental stress and limited nutrient supply. Different types of carbon, nitrogen, and phosphorus sources were investigated to achieve optimal PHA productivity. Novosphingobium sp. THA_AIK7 was cultured in a mineral salt medium (MSM) with various carbon, nitrogen, and phosphorus sources. Crude glycerol, monosodium glutamate (MSG), and Na₂HPO₄·7H₂O were the best sources for growth and PHA production. Biomass and PHAs derived from these nutrient sources were maximized at 5.23 g/L and 1.44 g/L, respectively with maximum PHA content 27.54%. Biomass and polymer productivity peaked in media containing Na₂HPO₄∙7H₂O at Q_x 0.073 ± 0.003 and Q_p 0.015 ± 0.000 g/L h. Experimental and predicted values from the logistic and Gompertz models trended in the same direction and accurately predicted microbial growth and PHA production. The extracted polymer structure was verified as a poly(-3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) copolymer by ¹H nuclear magnetic resonance (NMR) spectrometry analysis. The hydroxyvalerate (HV) monomer contents in the polymers derived from crude glycerol, fructose, and glycerol were 10.85, 10.16, and 6.59 mol%, respectively. Results indicated that Novosphingobium sp. THA_AIK7 produced a PHBV copolymer from various carbon sources without precursor addition.

How to Cite

Promdee, S., Areesirisuk, A. ., Posung , M. ., Tuntigumthon , S. ., Dhepakson, P., Imai, T., & Teeka, J. (2025). Effect of carbon, nitrogen and phosphorus on PHAs produced from Novosphingobium sp. THA_AIK7 and its structure. Asia-Pacific Journal of Science and Technology, 30(02), APST–30. https://doi.org/10.14456/apst.2025.18

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