Efficiency of Bacillus spp. isolated from food waste compost to control leaf spot disease in green oak leaf lettuce

Siriwiparat Phiakaew

Division of Biological of Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110

Jutarut Iewkittayakorn

Division of Biological of Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110

Usmana Meehae

Division of Biological of Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110

Sudarat Suwannarat

Division of Biological of Science, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla, 90110

Anurag Sunpapao

Agricultural Innovation and Management Division, Faculty of Natural Resources, Prince of Songkla University, Hatyai, Songkhla, 90110 Thailand

Juntima Chungsiriporn

Division of Chemical Engineering, Faculty of Engineering, Prince of Songkla University, Hat Yai, Songkhla, 90110

DOI: https://doi.org/10.14456/apst.2025.75

Keywords: Bacillus amyloliquefacien strain C2-1 Corynespora cassiicola dual culture assay leaf spot disease lettuce

Abstract

Bacillus spp. are often used as bioactive agents to control plant diseases. Corynespora cassiicola is a fungus that causes a serious disease of salad vegetables known as leaf spot disease. In this study, 08 bacterial strains belonging to Bacillus spp, isolated from food waste compost, were tested for their ability to induce hemolysis on sheep blood agar and to inhibit C. cassiicola in a dual culture assay. The 8 strains included B. subtillis strain BS, B. licheniformis strain No.13, B. amyloliquefqciens strain C2-1, B. tequilensis strain 1-BA, B. licheniformis strain 2-BA, Lysinibacillus sp. strain 3-BA, B. altitudinis strain No.20 and B. licheniformis LTWS strain No. 30. Two strains, B. altitudinis strain No. 20 and B. licheniformis LTWS strain No. 30 induced α-hemolysis, while the other 6 strains induced γ-hemolysis. Of the six non-hemolytic strains, B. amyloliquefaciens strain C2-1, B. licheniformis strain 2-BA and Lysinibacillus sp. strain 3-BA showed antifungal activity against C. cassiicola in the dual culture assay but B. amyloliquefaciens strain C2-1 inhibited C. cassiicola more strongly than the other two strains, with a percent inhibition of 69.38% after 9 days. B. amyloliquefaciens strain C2-1 was effective in suppressing the growth of C. cassiicola on the leaves of green oakleaf lettuce. The result of this study suggests that B. amyloliquefaciens strain C2-1 could be used as an alternative biocontrol agent against C. cassiicola.

How to Cite

Phiakaew, S. ., Iewkittayakorn, J., Meehae, U., Suwannarat, S., Sunpapao, A. ., & Chungsiriporn, J. (2025). Efficiency of Bacillus spp. isolated from food waste compost to control leaf spot disease in green oak leaf lettuce. Asia-Pacific Journal of Science and Technology, 30(05), APST–30. https://doi.org/10.14456/apst.2025.75

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