In vitro antioxidant capacity, total phenolic and flavonoid contents, and wound healing potential in Mangifera indica L. leaf extracts

Panthip Rattanasinganchan

Faculty of Medical Technology, Huachiew Chalermprakiet University, Samut Prakan, Thailand

Kittipat Sopitthummakhun

Faculty of Science and Technology, Huachiew Chalermprakiet University, Samut Prakan, Thailand

Wicharn Janwitayanuchit

Faculty of pharmaceutical science, Huachiew Chalermprakiet University, Samut Prakan, Thailand

DOI: https://doi.org/10.14456/apst.2025.63

Keywords: Mangifera indica L. Human keratinocyte wound healing activity maceration antioxidant capacity

Abstract

Mangifera indica L. is a rich source of biological active compounds, prominently Mangiferin, characterized by its glycosylated xanthone structure. This compound is abundant in various edible sources, such as mango, and exhibits diverse biological activities. Extensive investigation into Mangiferin has highlighted antioxidant, anti-inflammatory, antidiabetic, anticancer, and antimicrobial properties. In this study, Mangiferin extract was prepared through maceration in 80% ethanol, yielding 1,628 g from an initial dry leaf mass of 4,234 g of dried leaf material. The ethanolic extract showed notable torox equivalent antioxidant capacity (TEAC) values for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays, measured at 0.32 ± 0.03 and 0.56 ± 0.01 mM Trolox equivalents per gram dry material, respectively. The ferric reducing ability power (FRAP) value was determined to be 6.95 ± 0.40 mM FeSO4 per milligram dry material. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays conducted on human adult calcium temperature (HaCaT) cell line indicated that concentrations ranging from 3.90 to 31.25 µg/mL did not significantly impact cell viability. Evaluation of the wound healing of the Mangiferin ethanolic extract demonstrates a consistent decrease in wound area for treated cells compared to untreated controls, with significant decreases observed after 48 hours (p < 0.05). These findings support the antioxidant and wound healing properties of the Mangiferin ethanolic extract, underscoring its therapeutic potential in promoting wound repair.

How to Cite

Rattanasinganchan, P., Sopitthummakhun, K., & Janwitayanuchit, W. (2025). In vitro antioxidant capacity, total phenolic and flavonoid contents, and wound healing potential in Mangifera indica L. leaf extracts. Asia-Pacific Journal of Science and Technology, 30(04), APST–30. https://doi.org/10.14456/apst.2025.63

References

Dar A, Faizi S, Naqvi S, Roome T, Zikr-ur-Rehman S, et al. Analgesic and antioxidant activity of mangiferin and its derivatives: the structure activity relationship. Biol Pharm Bull. 2005;28(4):596–600.

Jose M, Varghese IV, Jayakar V, Lokapur V, Srinivasa K, Shantaram M. Evaluation of antioxidant activities and free radical scavenging properties in mango leaves, husks of areca and coconut. RJPBCS. 2018;9(4):1607-1619.

Garcia D, Delgado R, Ubeira FM, Leiro J. Modulation of rat macrophage function by the Mangifera indica L. extracts Vimang and Mangiferin. Int. Immuno pharmacol. 2002; 2:797-806.

Coelho EM, Olinda de Souza MEA, Corrêa LC, Viana AC, Cavalcanti de Azevêdo L, Lima MS. Bioactive compounds and antioxidant activity of mango peel liqueurs (Mangifera indica L.) produced by different methods of maceration. Antioxidants. 2019;8(4):102-112.

Andreu GP, Delgado R, Velho J, Inada NM, Curti C, Vercesi AE. Mangifera indica L. extract (Vimang) inhibits Fe2+-citrate-induced lipoperoxidation in isolated rat liver mitochondria. Pharmacol Res. 2005; 51:427-435.

Saha S, Sadhukhan P, Sil PC. Mangiferin: A xanthonoid with multipotent anti-inflammatory potential. BioFactors. 2016;42(5):459-474.

Espinosa-Espinosa L, Garduño-Siciliano L, Rodriguez-Canales M, Hernandez-Portilla LB, Margarita Canales-Martinez M, Rodriguez-Monroy MA. The wound-healing effect of mango peel extract on incision wound in a murine model. Molecules. 2022;27(1):259-277.

Jutiviboonsuk A, Sardsaengjun C. Mangiferin in leaves of three Thai mango (Mangifera indica L.) varieties. Isan J Pharm Sci. 2010;6(3):122-129.

Xie J, Schaich KM. Re-evaluation of the 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) assay for antioxidant activity. J Agric Food Chem. 2014;62(19):4251-4260.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999;26(9/10):1231-1237.

Narusawa U. General characteristics of sigmoidal model equation representing quasi-static pulmonary P-V curves. J Appl Physiol. 2001; 91:201-210.

Alam MN, Bristi NJ, Rafiquzzaman Md. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharm J. 2013; 21:143-152.

Langley-Evans SC. Antioxidant potential of green and black tea determined using ferric reducing power (FRAC) assay. Int J Food Nutr. 2000; 51:181-188.

Ainsworth EA, Gillespie KM. Estimation of total phenolic content and other oxidation substrates in plant tissues using folin-ciocalteu reagent. Nat Protoc. 2007;2(4):875-877.

Zhishen J, Mengcheng T, Jianming W. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem. 1999;64(4):555-559.

Jonkman JEN, Cathcard JA, Xu F, Bartolini ME, Amon JE, Stevens KM, et al. An introduction to the wound healing assay using live-cell microscopy. Cell Adhes Migr. 2014;8(5):440-451.

Prior RL, Wu X, Schaich K. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agri Food Chem. 2005; 53:4290‐ 4302.

Anbalagan K, Kumar MM, Ilango K, Mohankumar R, Priya RL. Prelusive scale extraction of Mangiferin form Mangifera indica leaves: Assessing solvent competency, process optimization, kinetic study, and diffusion modelling. Ind Crops Prod. 2019;140(5):11703-11710

Kawpoomhae K, Sukma M, Ngawhirunpat T, Opanasopit P, Sripattanaporn A. Antioxidant and neuroprotective effects of standardized extracts of Mangifera indica leaf. Thai J Pharm Sci. 2010; 34: 32-34.

Marinova D, Ribarova F, Atanassova M. Total phenolics and total flavonoids in Bulgarian fruits and vegetables. J Univ Chem Tech Metall. 2005; 40:255‑260.

Shamsudin NF, Ahmed QU, Mahmood S, Shah SAA, Khatib A, Mukhtar S, et al. Antibacterial effects of flavonoids and their structure-activity relationship study: A comparative interpretation. Molecules. 2022;27(4):1149-1192.

Yehia RS, Altwaim SA An insight into in vitro antioxidant, antimicrobial, cytotoxic, and apoptosis induction potential of mangiferin, a bioactive compound derived from manifera indica. Plants. 2023;12(1539):1-17.

Crozier A, Jaganath, IB, Clifford, MN. Dietary phenolics: Chemistry, bioavailability and effects on health. Nat Prod Rep. 2009;26(8):1001–1043.

Rao MA, Gianfreda L. Properties of acid phosphatase–tannic acid complexes formed in the presence of Fe and Mn. Soil Biol Biochem. 2000; 32:1921–1926.

Wu J-Y, Ding H-Y, Wang T-Y, Tsai Y-L, Ting H-J, Chang T-S. Improving aqueous solubility of natural antioxidant Mangierin through glycosylation by maltogenic amylase from Parageobacillus galactosidasius DSM 18751. Antioxidants. 2021;10(1817):1-16.

Allaw M, Pleguezuelos-Villa M, Manca ML, Caddeo1 C, Aroffu1 M, Nacher A.et al. Innovative strategies to treat skin wounds with Mangiferin: fabrication of transfer some modified with glycol and mucin. Nanomedicine (Lond.). 2020;15(17):1671-1685.

Daud NH, Aung CS, Hewavitharana AK, Wilkinson AS, Pierson JT, Roberts-Thomson SJ, et al. Mango extracts and mango component Mangiferin promote endothelial cell migration. J Agric Food Chem. 2010;58(8):5181-5186.

Lwin OM, Giribabu N, Kilari KE, Salleh N. Topical administration of mangiferin promotes healing of the wound of streptozotocin-nicotinamide-induced type-2 diabetic male rats. J Dermatolog Treat. 2021;32(8):1039-1048.

Yadav VP, Shukla A, Choudhury S, Singh R, Anand M, Prabhu SN. IL1β/ TNFα/COX-2/VEGF axis responsible for effective healing potential of C-glucoside xanthone (mangiferin) based ointment in immunocompromised rats. Epub. 2022; 158:156012.

Matkowski A, Piotr Kuś, Edyta Góralska, Dorota Woźniak. Mangiferin-abioactive xanthonoid, not only from mango and not just antioxidant. Mini Rev Med Chem. 2013;13(3):439-455.

Lee JY, Kim H, Moon Y, Kwak S, Kang CG, Park C, Jo J, Kim SW, Pal K, Kang DH, Kim D. Enhancement of the water solubility and antioxidant capacities of mangiferin by transglucosylation using a cyclodextrin glycosyltransferase. Enzyme Microb Technol. 2022; 159:110065.

Wu Y, Wang Y, Long L, Hu C, Kong Q, Wang Y. A spatiotemporal release platform based on pH/ROS stimuli-responsive hydrogel in wound repairing. J Control Release. 2022; 341:147-165.

Samadarsi R, Mishra D, Dutta D. Mangiferin nanoparticles fortified dairy beverage as a low glycemic food product: its quality attributes and antioxidant properties. Int J Food Sci Technol. 2020; 2:589-600.

Roberto CM. Mangiferin: A comprehensive review on its extraction, purification and uses in food systems. Adv. J Colloid Int Sci. 2024; 329:103188.