Euis Reni Yuslianti
Faculty of Dentistry, Universitas Jenderal Achmad Yani, Cimahi, 40531, Indonesia
Agus Susanto
Faculty of Dentistry, Universitas Padjadjaran, Sumedang, 45363, Indonesia
Afifah Bambang Sutjiatmo
Faculty of Pharmacy, Universitas Jenderal Achmad Yani, Cimahi, 40531, Indonesia
Wahyu Widowati
Faculty of Medicine, Maranatha Christian University, Bandung, 40164, Indonesia
Vini Ayuni
Biomolecular and Biomedicine Research Center, Aretha Medika Utama, Bandung, 40163, Indonesia
Dhanar Septyawan Hadiprasetyo
Faculty of Pharmacy, Universitas Jenderal Achmad Yani, Cimahi, 40531, Indonesia
Keywords: Antioxidant activities, Antibacterial activities, Apis mellifera, Royal jelly, Tooth health
Abstract
Inadequate care can cause oral health issues, leading to harmful bacteria–fungi interactions. Natural compounds, such as royal jelly, which is rich in polyphenols and flavonoids, may help reduce the risk of such problems. This study investigated the antioxidant and antibacterial potential of royal jelly, particularly its 10-hydroxy-2-decenoic acid (10-HDA) component, in preventing oral diseases. Royal jelly was evaluated through antioxidant assays, including 2,2-diphenyl-1-picrylhydrazyl (DPPH); 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS); nitric oxide (NO); and hydrogen peroxide (H2O2) scavenging tests, as well as antimicrobial assays using minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), and Kirby–Bauer disc diffusion methods. The antioxidant activity values were 107.67 ± 0.12 µg/mL DPPH, 234.63 ± 0.41 µg/mL (ABTS), 158.61 ± 0.25 µg/mL NO, and 210.24 ± 0.23 µg/mL H2O2. Disc diffusion tests showed inhibition against C. albicans (57.79%), P. gingivalis (56.64%), and S. mutans (53.00%). These findings indicate that royal jelly is a promising candidate for toothpaste formulation due to its antibacterial and antioxidant properties.
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